Poliovirus-mediated entry of pokeweed antiviral protein

Extracellular localization of pokeweed antiviral protein.

Pokeweed antiviral protein is an enzyme of Mr 29,000 known to inactivate a wide variety of eukaryotic ribosomes. We have used electron microscopy to show that the antibody specific for the protein is bound within the cell wall matrix of leaf mesophyll cells from Phytolacca americana. Any penetration or breakage of the cell wall and membrane could allow the enzyme to enter the cytoplasm, where i...

EFFECT OF POKEWEED ANTIVIRAL PROTEIN IN b COMBINATION WITH GUANIDINE ON POLIOVIRUS MACROMOLECULAR SYNTHESIS APPROVED: APPROVED: DEAN OF TIiE GRADUATE S C ~ O L EFFECT OF POKEWEED ANTIVIRAL PROTEIN IN COMBINATION WITH GUANIDINE ON POLIOVIRUS MACROMOLECULAR SYNTHESIS THESIS

Inhibition of pokeweed antiviral protein (PAP) by turnip mosaic virus genome-linked protein (VPg).

Pokeweed antiviral protein (PAP) from Phytolacca americana is a ribosome-inactivating protein (RIP) and an RNA N-glycosidase that removes specific purine residues from the sarcin/ricin loop of large rRNA, arresting protein synthesis at the translocation step. PAP is also a cap-binding protein and is a potent antiviral agent against many plant, animal, and human viruses. To elucidate the mechani...

Pokeweed Antiviral Protein, a Ribosome Inactivating Protein: Activity, Inhibition and Prospects

Viruses employ an array of elaborate strategies to overcome plant defense mechanisms and must adapt to the requirements of the host translational systems. Pokeweed antiviral protein (PAP) from Phytolacca americana is a ribosome inactivating protein (RIP) and is an RNA N-glycosidase that removes specific purine residues from the sarcin/ricin (S/R) loop of large rRNA, arresting protein synthesis ...

Seasonal variations in different forms of pokeweed antiviral protein, a potent inactivator of ribosomes.

Pokeweed antiviral proteins enzymatically inactivate the 60 S subunit of eucaryotic ribosomes in cell-free preparations. Three different species of the enzyme can be isolated from spring leaves, summer leaves, and seeds of pokeweed. Sequence analyses of the NH2-terminal residues show that pokeweed antiviral protein, isolated from spring leaves and seeds, are homologous and differ in 11 of the 2...